Simple and promising paper-based electrochemical platform for serological detection of American tegumentary leishmaniasis

BACKGROUND American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key. OBJECTIVES This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL. METHODS Platform was fabricated in Whatman N°1 paper, contains a hydrophobic zone generated by wax printing, two pencil graphite electrodes, and uses specific crude extracts (CA) antigens for ATL immuno-determination. The platform performance was analysed by measuring the relative impedance change for different antigen-antibody combinations. Then, 10 serum human samples previously diagnosed by the gold standard (five positive ATL cases and five non-ATL cases) were evaluated. FINDINGS The platform presented a linear response for the charge transfer resistance (ΔRct) and the interface reactance (ΔXc). Also, optimal working conditions were established (1/60 serum dilution and 180 µg/mL CA concentration). Then, the platform permits to distinguish between ATL and non-ATL (p < 0.05) human serum samples. MAIN CONCLUSIONS Our platform could allow the diagnosis, management, and monitoring of leishmaniasis while being an extremely simple and environmentally friendly technology.

Evaluation of the occlusal contact area of molar dental implants comparing two different thicknesses (16 µm and 200 µm) of articulating occlusal papers and forces (200 and 250 N): a pivot in vitro study

Introduction: The goal of this pilot study was to evaluate the differences between checking occlusion on implants crowns using 16 or 200 µm thickness of articulating occlusal paper, and to compare the stained occlusal area between the groups after bite forces of 200 and 250 N. Methods: It was included 10 casts of articulated-type IV gypsum, 10 NiCr crowns, articulating occlusal papers (16 µm and 200 µm thick), and a compression test machine. Compressive forces (200 and 250 N.mm) were applied on models, to check the occlusal contact area of fixed and cemented crowns. The contact areas on the crowns were measured through images obtained by the scanning electron microscope. Statistical tests were performed considering the significant level of 5% (p≤0.05). Results: The stains found using 200 µm of articulating paper were higher than those with 16 µm, independent of the force applied. However, the stains obtained in lower teeth with different strengths (200 and 250N) marked with 16 µm articulating paper were not possible to score. The articulating paper variable had significant statistical results (p=0.002), while the variables force (p=0.443) and articulating paper-force interaction (p=0.607) were not significant. The mean area found in staining using the 200 µm and 16 µm papers was, respectively, 8.3380 mm2 and 3.4759 mm2. Conclusion: It was possible to confirm that 200 µm of articulating occlusal paper showed better and significant results to stain the occlusal area, permitting a more accurate adjustment independent of the force applied.

Application of paper review combined with situational exercise in standardized training and teaching of ophthalmology nurses / 中华医学教育探索杂志

Objective:To investigate the application effect of paper review combined with situational exercise in the standardized training and teaching of ophthalmology nurses.Methods:A total of 39 ophthalmology nurses who received standardized training from September 2019 to July 2020 were selected as control group, and 42 ophthalmology nurses who received standardized training from September 2020 to July 2021 were selected as study group. The nurses in the control group received traditional teaching, and those in the study group received teaching with paper review and situational exercise. The two groups were compared in terms of the scores of theoretical knowledge and operation skills, comprehensive qualities (autonomous learning ability, independent analysis and problem-solving ability, nurse-patient communication ability, organization and coordination ability, and comprehensive first aid ability) before and after teaching, and the degree of satisfaction with teaching. SPSS 26.0 was used to perform the independent samples t-test, the paired t-test, the chi-square test, and the rank sum test. Results:After standardized training, compared with the control group, the study group had significantly higher scores of theoretical knowledge (93.29±1.82 vs. 90.36±1.51, P<0.05) and operation skills (95.14±1.34 vs. 92.62±1.26, P<0.05). After standardized training, both groups had significant increases in the scores of autonomous learning ability, independent analysis and problem-solving ability, nurse-patient communication ability, organization and coordination ability, and comprehensive first aid ability, and the study group had significantly higher scores of these comprehensive qualities than the control group ( P<0.05). There was a significant difference in the distribution of the degree of satisfaction with teaching between the two groups ( P<0.05), and the study group had a significantly higher degree of satisfaction than the control group. Conclusion:Paper review combined with situational exercise can improve the specialized theoretical knowledge, technical operational level, and comprehensive qualities of ophthalmology nurses receiving standardized training and enhance the degree of satisfaction with teaching.

Statistic of minimally invasive cardiovascular surgery of China in 2020—2021 and development in the future / 中国胸心血管外科临床杂志

@#Committee of Minimally Invasive Cardiovascular Surgery (CMICS) annually investigates the minimally invasive cardiovascular surgery performed by departments of cardiovascular surgery of all hospitals in China of last year, and makes classification and summary according to the operation amount of minimally invasive surgery, regional and hospital distribution, and publishes it on the theme report of China Minimally Invasive Cardiovascular Surgery Conference (CMC). In 2021, CMICS published the 2018-2019 annual data of Chinese cardiovascular surgery in the form of a white paper for the first time in the Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, which attracted great attention from peers. In this statistical report, CMICS will focus on the volume of minimally invasive cardiovascular surgery, regional and hospital distribution in China (excluding Hong Kong Special Administrative Region, Macao Special Administrative Region, and Taiwan Province) in the 2020—2021 for your reference.

Performance evaluation of colloidal gold test paper for Legionella pneumophila / 中国生物制品学杂志

@#ObjectiveTo prepare colloidal gold immunochromatographic test paper for rapid detection of Legionella pneumophila(LP)and test its performance to ensure that it meets the national clinical diagnostic standards.MethodsLP colloidal gold immunochromatographic test paper was prepared based on double antibody sandwich ELISA,and tested for the cross reactivity,anti-interference,sensitivity,hook effect,stability and other aspects.ResultsLP colloidal gold immunochromatography test paper showed no cross reaction with 22 common pathogens in respiratory tract such as Moraxella catarrhalis,and was not affected by internal and external interferences in respiratory tract;The minimum detection limit for LP was 2.00 × 105cfu/mL,with good sensitivity and no hook effect;Under the conditions of accelerated aging at 45 ℃,simulated high temperature transportation and frozen transportation,the repeatability and stability of test paper were not affected,and the stability was good in the same batch and between different batches.ConclusionThe prepared LP colloidal gold immunochromatographic test paper realized rapid detection of LP,which was simple to operate and had good application prospect and popularization value.

Detection of IgG protein in human urine based on vertical flow paper microfluidic chip / 生物工程学报

The kidney is the body's most important organ and the protein components in urine could be detected for diagnosing certain diseases. The amount of IgG protein in urine could be used to determine the degree of kidney function damage. IgG protein in human urine was detected by vertical flow paper-based microfluidic chip, double-antibody sandwich immunoreaction, and cell phone image processing. The results showed that using an IgG antibody concentration of 500 μg/mL and a gold standard antibody concentration of 100 μg/mL, the image signal showed a good linear relationship in the range of IgG concentration of 0.2-3.2 μg/mL, with R2=0.973 3 achieved. A complete set of detection devices were designed and the detection method showed good non-specificity.

Adjustable "paper clip" techniques suturing the dorsal vein complex in radical prostatectomy / 现代泌尿外科杂志

【Objective】 To investigate the efficacy of the adjustable "paper clip" techniques in the suture of dorsal vein complex (DVC) and retention of urethral function in robot-assisted laparoscopic radical prostatectomy (RALRP). 【Methods】 A total of 30 cases of prostate cancer treated with RALRP were enrolled, all of which used the adjustable "paper clip" techniques. During operation, the DVC was sewed with barbed suture, and then a reverse suture was made through two sides of the prostatic ligaments. A Hem-o-lock was used to fasten the suture, which would be flexible to control the degree of tightness for the ligature. Perioperative and follow-up data of urinary continence and symptoms were collected and analyzed. 【Results】 All operations were successful. The estimated blood loss was (123.3±80.7) mL, 53.6% patients recovered continence in 1 month, and the continence rate increased to 92.9% and 96.3% at month 3 and 6. 92.9 of patients had no risk of incontinence 3 months after surgery. 【Conclusion】 The adjustable "paper clip" techniques have advantages in reducing blood loss, maintaining clear surgical field, preserving urethral function, and improving urinary continence.

Practice of paper-free whole blood collection system in constructing intelligent blood station / 中国输血杂志

【Objective】 To establish a paper-free system of the total whole blood donation flow in constructing intelligent blood stations, and build digitalized whole blood donation system for practice. 【Methods】 A paper-free whole blood collection system was constructed through information process reforming, system frame designing and data network transportation constructing, and was applied in various blood donation scenario. 【Results】 Fixed blood collection sites carried out 49 063 donations via paper-free information system from November 2022 to July 2023, and 24 822 donations( group blood donation) were conducted via paper-free system from April to July 2022. Compared with the traditional paper-based model, paper-free system is safer, more standardized and more convenient, effectively enhancing the experience of blood donors. 【Conclusion】 The construction of paper-free whole blood collection system effectively enhances the experience of blood donors, improves the safety, accuracy, traceability of the data, and has good social value and economic value, which is worth popularizing.

Electronic versus Paper-Based Data Collection for Conducting Health-Care Research: A Cost-Comparison Analysis

Background: Containing expenditure and efficient resource use is essential to limit the increasing costs of health research. Electronic data collection (EDC) is thought to reduce the costs compared to paper-based data collection (PDC). Objectives: As economic evidence in this area is scanty, especially in low- and middle-income countries, the objectives of the study are to perform an economic evaluation and compare the cost between EDC and PDC. Methods: A cost-comparison study was conducted to compare between EDC and PDC from the institutional perspective for the year 2018, based on a community?based survey. Step?down cost accounting was adopted with a bottom?up approach for cost estimation. Total and unit costs were estimated with the base case comparison between EDC and PDC while using SPSS software (e?SPSS and p?SPSS, respectively). We conducted scenario analyses based on the usage of different software, R and STATA for both EDC and PDC (e-R, p-R, e-STATA, and p-STATA, respectively). One-way and probabilistic sensitivity analysis (PSA) was performed to examine the robustness of the observed results. Results: In the base-case analysis, total costs of EDC and PDC were ?72,617 ($1060.9) and 87,717 ($1281.5), respectively, with estimated cost reduction of ?15,100 ($220.6). In other scenarios, the estimated cost reduction for e?R, e-STATA, p-R, p-STATA was ??274 ($4.0), 98 ($1.4), 14826 ($216.6), and 15,002 ($219.2), respectively, when compared to EDC?SPSS. On one-way and PSA, the results of the cost-comparison analysis were robust. Conclusion: EDC minimizes institutional cost for conducting health research. This finding will help researchers in efficiently planning for the budget for their research.

Rol crítico del trabajo final de graduación en el proceso de formación investigativa del estudiantado de ciencias del movimiento humano

Rojas-Valverde, D. (2022). Rol crítico del trabajo final de graduación en el proceso de formación investigativa del estudiantado de ciencias del movimiento humano. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(1), 1-10. Las prácticas científicas son un pilar en la formación de los profesionales de las Ciencias del Movimiento Humano (CMH), lo que acerca al estudiantado a la identificación y solución de problemas del contexto. Si bien las experiencias prácticas e investigativas son inherentes al proceso de mediación y formación pedagógica, un factor vinculado con este proceso es la elaboración del trabajo final de graduación (TFG). Gracias a los procesos de autoevaluación de las carreras, se ha identificado que el TFG es uno de los factores por los cuales la eficiencia terminal es cada vez menor. Esta problemática se ha subsanado, entre otras formas, con la utilización de modalidades como la prueba de grado, que requieren de menos tiempo y son muy puntuales en el mismo factor. Lo anterior si bien solventa en cierta medida la necesidad de aumentar las tasas de eficiencia terminal, no es considerado el mejor ejercicio académico para la identificación y resolución de problemas del contexto entorno a las CMH. En este sentido, urge resignificar el TFG como un proceso de enseñanza-aprendizaje de los conocimientos, valores y habilidades vinculados al ejercicio de la profesión y no reducirlo a una tarea al final de la etapa educativa. Para ello, debe vincularse el TFG a las prácticas de los cursos, provocando una construcción gradual del proceso investigativo y, de esta forma, implementar planes de diseño, gestión y seguimiento de los TFG.

Rojas-Valverde, D. (2022). The critical role of the final graduation paper in the process of research formation of the students of human movement science. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(1), 1-10. Scientific practices are a pillar in the formation of professionals in Human Movement Science (HMS), which brings students closer to identifying and solving context problems. While practical and research experiences are inherent to the process of pedagogic mediation and formation, a factor linked to this process is the development of the final graduation paper (FGP). Thanks to the self-evaluation processes in study curricula, it has been possible to identify that the FGP is one of the factors why terminal efficiency is increasingly lower. This problem has been remedied, among other ways, with the use of other evaluation modes such as the degree exam, which requires less time and is very on-spot for the same factor. While this meets to a certain extent the need to increase the rates of terminal efficiency, it is not considered to be the best academic exercise for the identification and solution of context problems related to HMS. In this respect, it is urgent to re-signify the FGP as a process of teaching/learning of the knowledge, values and skills related to the practice of this profession, instead of reducing it to a homework at the end of the educational stage. For this purpose, it is necessary to link the FGP to course practices, resulting in a gradual construction of the research process, and thus implementing the plans for the design, management and follow-up of the FGP.

Rojas-Valverde, D. (2022). Papel crítico do trabalho final de graduação no processo de formação investigativa dos estudantes de ciências do movimento humano. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 20(1), 1-10. As práticas científicas são um pilar na formação dos profissionais de Ciências do Movimento Humano (CMH), aproximando os estudantes da identificação e solução de problemas contextuais. Embora as experiências práticas e investigativas sejam inerentes ao processo de mediação e formação pedagógica, um fator vinculado a esse processo é a elaboração do Trabalho de Conclusão de Curso (TCC). Graças aos processos de autoavaliação das matérias, identificou-se que o TCC é um dos fatores pelos quais a eficiência terminal é cada vez menor. Essa problemática foi remediada, entre outras formas, com a utilização de modalidades como a prova de graduação, que precisam de menos tempo e são muito pontuais no mesmo aspecto. As considerações anteriores, embora resolvam, em certa medida, a necessidade de aumentar as taxas de eficiência terminal, não é considerado o melhor exercício acadêmico para a identificação e resolução de problemas contextuais relacionados com as CMH. Nesse sentido, é improrrogável ressignificar o TCC como um processo de ensino-aprendizagem dos conhecimentos, valores e habilidades vinculados ao exercício da profissão e não o reduzir a uma tarefa no final da etapa educativa. Para isso, é preciso vincular o TCC às práticas dos cursos, promovendo uma construção gradativa do processo investigativo e, assim, implementar planos de elaboração, gestão e acompanhamento do TCC.

Exploration on writing features and learning strategies of English biomedical research papers / 中华医学教育探索杂志

Writing and publishing biomedical research papers in English for academic purposes (EAP) has become an essential requirement for medical postgraduates in China. To face this challenge, medical postgraduates need adapt themselves to the local environment of English as a second language and the transformation from writing for examinations to writing for application. This change determines the features of biomedical research papers, including being application-oriented, using clear and concise language, organizing the discourse by specific genre rules, and assessing the quality according to the completion of communicative purposes. Based on these features, students can exploit the learning strategies of choosing a word suited for the style, structuring a sentence according to the logic, creating a paragraph of continuity and one main idea, and composing the discourse in line with the genre. These strategies may help medical postgraduates effectively improve their ability to write biomedical research papers in English.

Application value of paper towel combined with 360° horizontal turning over method in preoperative preparation of gastroscopy / 中国实用护理杂志

Objective:To investigate the effect of paper towel combined with 360° horizontal turning over method on the field of vision, operation time and detection of small lesions before gastroscopy, so as to improve the detection rate of early gastrointestinal cancer.Methods:The outpatients and inpatients who voluntarily accepted gastroscopy in the First Affiliated Hospital of Shandong First Medical University from May to October 2020 were enrolled. A prospective randomized controlled study was used. They were divided into the experimental group (paper towel group combined with 360° horizontal turning over) and the control group (routine) according to the random number table. Finally, a total of 948 patients were included, including 487 cases in the experimental group and 461 cases in the control group. The visual field clarity, operation time, rinse times, detection of micro lesions and detection of early cancer of the two groups were compared.Results:The scores of gastric mucosa clarity (fundus, body, antrum and whole stomach) in experimental group were (1.61 ± 0.79), (1.18 ± 0.56), (1.01 ± 0.36), (3.20 ± 0.51) points, which were significantly lower than (2.56 ± 0.82), (2.01 ± 0.65), (1.98 ± 1.10), (5.05 ± 0.89) points in control group. The difference was statistically significant ( t values were 0.02-0.07, all P<0.05). The operation time of the experimental group was (5.91 ± 0.41) min, which was significantly shorter than (6.80 ± 0.72) min of the control group, and the difference was statistically significant ( t=-7.46, P<0.05). The number of flushing in the experimental group was 67 times, accounting for 13.8%, which was significantly lower than 144 times in the control group, accounting for 31.2%, and the difference was statistically significant ( χ2=54.78, P<0.05). The number of microlesions in the experimental group was 398 cases, which was higher than 245 cases in the control group. The difference was statistically significant ( χ2=8.43, P<0.05). For biopsy pathology, the detection of precancerous lesions or early carcinomas in the experimental group (56 cases) was significantly higher than that in the control group (24 cases), and the difference was statistically significant ( χ2=6.45, P<0.05). Conclusions:The application of paper towel combined with 360° horizontal turning over method before gastroscopy can significantly improve the clarity of gastroscope field of vision, shorten the operation time, increase the detection rate of small lesions, and effectively improve the detection rate of early gastrointestinal cancer, which is a simple and practical preoperative preparation method.

Bibliometric and visual analysis of Chinese-language scholarly journal papers published by China provincial centers for disease control and prevention from 2011 to 2020：based on CSCD data / 环境与职业医学

Background By analyzing the publication of scientific and technological papers of the provincial centers for disease control and prevention (CDCs) in China, we can understand their scientific research strength, dominant disciplines, research hotspots, and talent development, which is conducive for further optimizing the scientific research construction of the disease control system. Objective To conduct a bibliometric analysis and draw a visual map of the Chinese Science Citation Database (CSCD) sourced papers published by 31 provincial CDCs in China from 2011 to 2020, to understand the scientific research status of China's provincial CDC system in the past ten years. Methods In April 2021, China National Knowledge Infrastructure was used to retrieve CSCD sourced papers published by 31 provincial CDCs (excluding Taiwan Province, Hong Kong and Macau Special Administrative Regions) in China as the primary unit from 2011 to 2020, and finally 5231 CSCD papers were included. Bibliometric analysis indicators include number of CSCD papers published and financial support rate, proportion of CSCD papers to total Chinese papers, distribution of CSCD papers published in high-volume journals, and number of papers for a specific CDC that their first authors affiliated with. Citespace 5.8.R1 software was used to draw author's cooperation network map, keyword co-occurrence map, keyword clustering information table, and keyword emergence map. Results The number of CSCD papers published every year showed a stationary trend, and the total funding rate was 61%, showing an year-by-year upward trend (increased from 49.3% in 2011 to 68.7% in 2020). Jiangsu Province, Fujian Province, and Beijing CDCs ranked the top three in the total number of CSCD papers contributed by a specific CDC. The Chinese Journal of Epidemiology, Chinese Journal of Public Health, Chinese Journal of Zoonoses, Chinese Journal of Preventive Medicine, and Chinese Journal of Endemic Diseases were the top five high-volume journals. The top three authors of CSCD papers (counted as co-authors) were Xu Bianli of Henan Provincial Center for Disease Control and Prevention (110 papers), Yan Yansheng of Fujian Provincial Center for Disease Control and Prevention (72 papers), and Wang Quanyi of Beijing Center for Disease Prevention and Control (71 papers), and they studied the epidemiology of parasitic diseases and infectious diseases, the prevention and treatment of natural epidemic diseases and AIDS, and the epidemiology of infectious diseases, respectively. The keyword co-occurrence map showed that AIDS, zoonotic diseases, hand-foot-mouth disease, etc. (frequency ≥90) were the key research directions of provincial CDCs in the past ten years. The keyword clustering categories were closely related, although there were subtle differences, the topics focused on virus and chronic disease research. The keyword emergence results suggested that the frontiers of research had gradually shifted from infectious diseases such as enterovirus, hantavirus, and Zika virus to food-borne diseases and chronic non-communicable diseases such as tumors and senile diseases over time. Conclusion The number of papers issued by the provincial CDCs in China in the past ten years has shown an overall steady and rising trend. The leading themes of researchers and institutions are infectious diseases, parasitic diseases, etc., while food-borne diseases and elderly diseases are the hot frontiers.

Analysis of SCI paper output and research hotspots of Chinese dental research institutions / 中华医学科研管理杂志

Objective:To statistically analyze SCI paper output and research hotspots of Chinese stomatology research institutes from 2011 to 2020.Methods:The papers of Chinese stomatology research institutions in the Web of Science database were searched and analyzed by bibliometric methods in various dimensions including publication time, institutional and journal distribution, international (regional) cooperation, and research hotspots.Results:The number and quality of publications in Chinese dental research institutions have increased significantly in the past decade, and the top four institutions in terms of publication number were Sichuan University, Peking University, Shanghai Jiaotong University, and Air Force Military Medical University. The distribution of the journals to which the articles belong was relatively scattered, and the research hotspots mainly focused on squamous cell carcinoma, periodontitis, bone regeneration, and materials science.Conclusions:The output of SCI papers and the number of high-quality papers in Chinese stomatology research institutions increased year by year during 2011-2020, and the scientific research strength continued to increase.

Analysis of articles and citation indexes in Chinese Journal of Behavioral Medicine and Brain Science / 中华行为医学与脑科学杂志

Objective:To analyze the changing trend of articles, authors and citation indexes in Chinese Journal of Behavioral Medicine and Brain Science(CJBMBS), so as to provide basis and guidance for further academic quality and editing and proofreading quality improving, and striving to build high-quality scientific and technological journal.Methods:Chinese Medical Journal Network and Wanfang database were used to obtain the information of articles published from 2009 to 2021.The citation indexes of the CJBMBS and the discipline journals of " neurology and psychiatry" were obtained from the natural science volume of the Citation Report (Core Edition) of Chinese Scientific and Technological Journals from 2002 to 2021.All data were analyzed by Excel 2010.Results:The CJBMBS published 187-314 articles per year from 2015 to 2021, and the annual number of articles decreased year by year.The average number of pages per article was 3.66-6.13 pages/article, showing an increasing trend year by year.Since 2015, the CJBMBS set up 3-7 key topics every year, and published 6.4%-16.2% articles of the annual.From 2009 to 2020, the average citation volume of magazine articles was 12.8-27.9, showing an increasing trend year by year.The average number of authors per article was basically stable at 4-6.From 2001 to 2020, the core impact factor of the CJBMBS was 0.188-1.327, with a wave like change and an overall upward trend.The total frequency of core citations was 155-2 836, with a rapid increase from 2001 to 2009, a stable at 2 500 from 2007 to 2017, and a downward trend after 2014.Compared with average level of the core journals of the discipline " neurology and psychiatry" , the core impact factor and the core total citation frequency of the CJBMBS from 2009 to 2020 were both significantly higher.From 2009 to 2020, the fund paper ratio of CJBMBS was 0.42-0.84, showing an upward trend.Conclusion:The citation indexes of CJBMBS are in the forefront of the discipline in recent years.In the future, we should pay more attention to the academic quality of papers and further expand the influence of the journal.

Current norms and practices in using a seizure diary for managing epilepsy: A scoping review

Epilepsy is a chronic and debilitating condition affecting people of all ages in many nations. Healthcare practitioners look for effective ways to track patients' seizures, and a seizure diary is one of the methods used. This scoping review sought to identify current norms and practices for using seizure diaries to manage epilepsy. Method: A scoping review was performed by screening relevant studies and identifying themes, categories and subcategories. Results: A total of 1125 articles were identified from the database; 46 full-text articles were assessed for eligibility, of which 23 articles were selected. The majority (48%) of the studies were prospective studies. The majority (65%) of the articles were studies conducted in the United States. The themes identified were types of seizure diaries used in clinical practice, contents and structure of a standardized seizure diary, the use and efficacy of seizure diaries in medicine and challenges relating to using a seizure diary for patient management. Conclusion: The study revealed that a seizure diary remains a relevant tool in managing epilepsy. The two forms of diaries in use are electronic and paper-based diaries. The high cost of data and the expensive devices required to access electronic diaries make it unsuitable in a resource-limited setting. Despite its disadvantages, imperfections and inadequacies, the paper-based diary is still relevant for managing patients with epilepsy in resource-limited settings. Contribution: This study reviewed the literature to find the current norms and practices in using seizure diaries. The benefits of the different formats were emphasized

Investigation and analysis on the cognition of scientific research integrity of authors of scientific and technological papers in a grade A tertiary hospital / 中华医学科研管理杂志

Objective:To understand the cognition of scientific research integrity of the authors of scientific and technological papers in a grade A tertiary hospital, the necessity and relevant needs of carrying out scientific research integrity construction, and provide a reference for improving the construction of relevant scientific research integrity system in the hospital.Methods:A questionnaire was used to survey 746 people who had published scientific and technological papers in a grade A tertiary hospital from 2016 to 2021, and the influencing factors of scientific research integrity cognition and construction were analyzed via a generalized linear model.Results:The overall awareness rate of scientific research integrity cognition of scientific papers was 76.72%, and the overall necessity rate of scientific research integrity construction was 77.75%. Generalized linear model analysis showed that other degrees refer to a doctor's degree, associate senior title, intermediate title, refer to junior title, or no title were negatively correlated with the cognition of scientific research integrity ( P<0.05), other ages refer to ≤30 years old, middle-level and above positions refer to no position, postgraduate mentor refer to non-postgraduate mentor, senior title refer to junior title or no title, postgraduate, nursing, administrative and research personnel refer to medical technology and pharmacy personnel, and presiding projects refer to no presiding project experience were positively related to scientific research integrity cognition ( P<0.05). Bachelor′s degree and no degree refer to doctor′s degree, associate senior and intermediate titles refer to junior title or no title were negatively related to the necessity of scientific research integrity construction ( P<0.05), middle level and above positions refer to no position, postgraduate, nursing and research personnel refer to medical technology and pharmacy personnel, were positively related to the necessity of scientific research integrity construction ( P<0.05). Behaviors with less than 60% cognition of scientific research integrity included the ethical problems of artificial intelligence, the ethical problems of stem cells, the problems of human genetic resources, and the definition of unauthorized human living drug and medical technology experiments. Conclusions:The hospital will take publicity and education as the basis, system norms as the criteria, daily supervision as the starting point, evaluation system as the core, carry forward the spirit of scientists, improve and implement the construction of the hospital's scientific research integrity system and supervision system, strengthen the integrity management of the whole process of scientific research activities, and guide medical personnel to practice excellent scientific research style and style of study.

IgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis / Avidez de IgG em amostras coletadas em papel filtro: Importância no diagnóstico precoce da toxoplasmose congênita

Abstract Objective The purpose of the present study is to standardize and evaluate the use of the immunoglobulin G (IgG) antibody avidity test on blood samples from newborns collected on filter paper to perform the heel test aiming at its implementation in ongoing programs. Methods Blood samples from newborns were collected on filter paper simultaneously with the heel prick test. All samples were subjected to immunoglobulin M IgM and IgG enzyme-linked immunosorbent assays (ELISA). Peripheral blood was collected again in the traditional way and on filter paper from newborns with high IgG levels (33). Three types of techniques were performed, the standard for measuring IgG in serum, adapted for filter paper and the technique of IgG avidity in serum and on filter paper. The results of the avidity test were classified according to the Rahbari protocol. Results Among the 177 samples, 17 were collected in duplicate from the same child, 1 of peripheral blood and 1 on filter paper. In this analysis, 1 (5.88%) of the 17 samples collected in duplicate also exhibited low IgG avidity, suggesting congenital infection. In addition, the results obtained from serum and filter paper were in agreement, that is, 16 (94.12%) samples presented high avidity, with 100% agreement between the results obtained from serum and from filter paper. Conclusion The results of the present study indicate that the avidity test may be another valuable method for the diagnosis of congenital toxoplasmosis in newborns.

Resumo Objetivo O objetivo do presente estudo é padronizar e avaliar a utilização do teste de avidez de anticorpos imunoglobulina G (IgG) em amostras de sangue de recémnascidos (RNs) coletadas em papel filtro para a realização do teste do pezinho visando a implementação nos programas já vigentes. Métodos Foram coletadas amostras de sangue de recém-nascidos em papel filtro simultaneamente ao teste do pezinho. Em todas as amostras, foram realizados os testes imunoenzimáticos (ELISA) imunoglobulina M (IgM) e IgG. Dos RNs que apresentaram altos índices de IgG (33), foi novamente coletado sangue periférico da forma tradicional e em papel filtro. Foram realizadas técnicas padrão para a dosagem de IgG em soro, adaptadas para papel filtro, e a técnica de avidez de IgG em soro e em papel filtro. Os valores obtidos para o teste de avidez foram classificados de acordo com o protocolo de Rahbari. Resultados Dentre as 177 recoletas, em 17 amostras foi realizada a coleta simultânea de sangue periférico e papel filtro da mesma criança. Nesta análise, 1 (5,88%) das 17 amostras coletadas em duplicata obteve também baixa avidez de IgG, sugerindo infecção congênita da criança, e houve concordância entre os resultados obtidos em soro e em papel filtro: 16 (94,12%) das amostras apresentaram alta avidez, com concordância de 100% entre os resultados obtidos em soro e em papel filtro. Conclusão Os dados do presente trabalho evidenciam que o teste de avidez poderá ser mais um método valioso a ser utilizado no diagnóstico da toxoplasmose congênita em RNs.

Light quality and dormancy overcoming in seed germination of Echium plantagineum L. (Boraginaceae) / Qualidade da luz e superação de dormência na germinação de sementes de Echium plantagineum L. (Boraginaceae)

Abstract Light is considered a factor that influences the seed germination of many weed species, and it can signal whether the environmental conditions are favorable or are not favorable for germination. We aimed to study if there is an influence of light quality and dormancy overcoming in seed germination of Echium plantagineum L. We carried out a 2 x 6 factorial experiment, with and without dormancy overcoming with potassium nitrate followed by immersion in gibberellic acid; six light qualities, obtained through the light filters: blue, green, red, far-red, white light and absence of light. The evaluations performed were germination speed index (GSI), average germination time (AGT), germination at the four and 14 days after seeding (DAS), accumulated germination and relative frequency of germination. We observed significant interaction among the light qualities and seed dormancy overcoming or not for the studied variables. There was no significant effect of light qualities, in the evaluated variables, when performing dormancy overcoming, presenting germination above 90% in all the light qualities. However, without dormancy overcoming, we observed greater GSI, germination at four and 14 DAS for the red light filter with 5, 4, 29 and 45%, respectively. When the seeds were submitted to the absence of light, and without dormancy overcoming, there was only 7% of germination at 14 DAS. The seeds of E. plantagineum presented greater germination under incidence of red light, without dormancy overcoming, being classified as preferably positively photoblastics, provided that the dormancy is not overcome.

Resumo A luz é considerada um fator que influencia a germinação das sementes de muitas espécies de plantas daninhas, podendo sinalizar se as condições ambientais são favoráveis ou não para a germinação. Objetivou-se estudar se há influência da qualidade da luz e superação de dormência na germinação de sementes de Echium plantagineum L. Realizou-se um experimento fatorial 2 x 6, com e sem superação de dormência com nitrato de potássio seguido pela imersão em ácido giberélico; seis qualidades de luz, obtidas através de filtros de luz: azul, verde, vermelho, vermelho-distante, luz branca e ausência de luz. As avaliações realizadas foram índice de velocidade de germinação (IVG), tempo médio de germinação (TMG), germinação aos quatro e 14 dias após a semeadura (DAS), germinação acumulada e frequência relativa de germinação. Observou-se interação significativa entre as qualidades de luz e a superação ou não de dormência das sementes para as variáveis estudadas. Não houve efeito significativo das qualidades de luz, nas variáveis avaliadas, ao realizar superação de dormência, apresentando germinação acima de 90% em todas as qualidades de luz. Todavia, sem superação de dormência, observou-se maior IVG, germinação aos quatro e 14 DAS para o filtro de luz vermelha com 5,4, 29 e 45%, respectivamente. Quando as sementes foram submetidas à ausência de luz, e sem superação de dormência, houve apenas 7% de germinação aos 14 DAS. As sementes de E. plantagineum apresentam maior germinação sob incidência de luz vermelha, sem superação de dormência, sendo classificadas como fotoblásticas positivas preferenciais, desde que não seja superada a dormência.

Caracterización química, degradabilidad ruminal y aceptabilidad por bovinos, de un subproducto de la industria del papel / Chemical characterization, ruminal degradability and bovine acceptability of a byproduct of the paper industry

RESUMEN Las variaciones climáticas pueden limitar la disponibilidad y la calidad de los forrajes. Se estudió un subproducto del papel (SIP) como alternativa a los forrajes en la ración de los bovinos. Se analizaron 12 muestras de SIP, para determinar su composición nutricional y degradabilidad ruminal. La aceptabilidad (1,07kg MS) por los bovinos de los SIP solo o en mezcla, se evaluó en 12 novillas Holstein, asignadas a dos cuadrados latinos, cada uno con tres tratamientos y dos réplicas. En el primer cuadrado, los tratamientos fueron SIP solo (T1); SIP+glicerol (T2) y SIP+melaza (T3). En el segundo, los tratamientos fueron SIP+suplemento comercial (SC) (T4); SIP+SC+glicerol (T5) y SIP+SC+melaza (T6). Los SIP presentaron bajos contenidos de PC (1,5±0,14%) y altos niveles de FDN (56,9±6,17%) y cenizas (53,3±1,98%) y una DIVMO (47,7±5,8%) media. Las concentraciones de P (0,045±0,006%) y K (0,023±0,03%) fueron bajas, mientras que las de Ca (13,5±0,8%), Cu (63,9±11,5%) y Fe (1365±189ppm) fueron altas. La tasa de desaparición de la MS del SIP fue de 11,8%*h-1; la fracción soluble ( a ), 14% y la fracción potencialmente degradable y no soluble ( b ), 46,6%. La aceptabilidad del SIP fue mayor, cuando se ofreció en mezclas (T1 vs T2-T3; p<0,05) y con concentrado y melaza (T4-T5 vs T6; p<0,05). La variación en la calidad composicional del SIP fue menor para la mayoría de nutrientes y su calidad baja, debido a una digestibilidad media, una baja PC y altas cenizas. Además, su aceptabilidad fue pobre cuando se suministró sola, pero mejoró con el uso de palatabilizantes o SC.

ABSTRACT Climatic variations can limit the availability and quality forages. The use of byproducts of the paper industry (BPI) was studied as part of cattle rations. Twelve samples of BPI were analyzed to determine the nutritional composition and ruminal degradability. The acceptability (1.07kg DM) of the BPI was evaluated in 12 Holstein heifers, assigned to two Latin squares with two replicates and three treatments each. The first square, the treatments were BPI alone (T1), BPI +glycerol (T2) and BPI +molasses (T3). In the second square, treatments were BPI +commercial supplement (CS) (T4), BPI +CS +glycerol (T5) and BPI + CS + molasses (T6). The BPI presented low CP (1.5±0.14%), high levels of NDF (59.6±6.17%) and ash (53.3±1.98%) and médium IVODM (47.7±5.8%). Concentrations of P (0.045±0.06%) and K (0.023±0.03%) were low while Ca (13.5±0.8%), Cu (63.9±11.5ppm) and Fe (1365±189ppm) high. Rate of DM disappearance for BPI was 11.8%*h-1, soluble fraction ( a ) 14% and a potentially degradable fraction but not soluble ( b ) 46.6%. The acceptability of BPI was higher when fed in mix (T1 vs T2-T3; p<0.05) and with concentrate and molasses (T4-T5 vs T6; p<0.05). Variation in nutritional composition of BPI was low for most and its nutritional quality low due to a moderate digestibility, a low CP and high ash. In addition, its acceptability was low but improved when supplied with the use of palatability agents and CS.